产品描述

PhoSL-hIgG1 是一种与人 IgG 恒定区融合的、与岩藻糖专一结合的凝聚素(fucose-specific lectin, specifically binding to fucose)，仅与 α1-6 岩藻糖基化的 N-聚糖特异性结合，而不与其他类型的岩藻糖基化的寡糖结合，例如 α1-2-、α1-3- 和 α1-4-岩藻糖基化聚糖。PhoSL与hIgG1 Fc的融合蛋白本身的聚糖中不含任何岩藻糖，从而大大提高了其结合特异性，可用作诊断的优良试剂。PE 标记的 PhosL 是人 IgG1 亚型。

PhoSL-hIgG1 is a lectin fused to human IgG constant region and specifically binds to α1-6-fucosylated N-glycans only and not other types of fucosylated oligosaccharides, such as α1-2-, α1-3-, and α1-4-fucosylated glycans. The fusion protein of PhoSL with hIgG1 Fc itself is devoid of any fucose in its glycan, thus dramatically increases its binding specificity, and can be used as an excellent reagent for diagnosis. PE labeled PhosL is human IgG1 isotype.

名称：PE PhoSL 凝聚素

货号：ATJ-5002

规格：100T

Figures above use PE PhosL 5 µl for 100 µl staining volume per 1 million cells. (left) is unstained cells.  PE PhosL stained wild type CHO (Middle), and afucosylated CHO cells (right).

细胞株名称：表达PhoSL-hIgG1凝聚素融合蛋白的细胞株

货号：ATJ-5002C

规格：5 x 10E6 cells

参考文献 References

1. Yuka Kobayashi, et al. A novel core fucose-specific lectin from the mushroom Pholiota squarrosa. J. Bio Chem, 2012 Oct 5;287(41):33973-82. https://pubmed.ncbi.nlm.nih.gov/22872641/

2. Kazuhiko Yamasaki, et al. Core fucose-specific Pholiota squarrosa lectin (PhoSL) as a potent broad-spectrum inhibitor of SARS-CoV-2 infection. FEBS J. 2023 Jan;290(2):412-427. https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16599

3. Tsunenori Ouchida, et al. The specific core fucose-binding lectin Pholiota squarrosa lectin (PhoSL) inhibits hepatitis B virus infection in vitro. Scientific Reports 2023 April; 13: 6175 https://www.nature.com/articles/s41598-023-28572-6